Biology and Biotechnology of Environmental Stress Tolerance in Plants (Aryadeep Roychoudhury)

Biology and Biotechnology of Environmental Stress Tolerance in Plants, Volume 3: Sustainable Approaches for

Enhancing Envir

l Stress Tolerance. Aryadeep Roychoudhury (Ed.)

with CRC Press (Taylor & Francis)

onmenta

published

demic Press, Inc. Co-

CHAPTER 14

Advances in Metabolomics Research in

Environmental Stress Response in Plants

ANKUR SINGH and ARYADEEP ROYCHOUDHURY^*

Post-Graduate Department of Biotechnology, St. Xavier’s College

(Autonomous), 30, Mother Teresa Sarani, Kolkata – 700016,

West Bengal, India

*Corresponding author. E-mail: [email protected]

ABSTRACT

Abiotic stress is one of the major limiting factors that significantly hampers

the development of plants and also negatively affects the quality and

quantity of crop yield. To overcome the negative effects of abiotic stresses,

plants reprogram their metabolic pathway which in turn enhances the level

of primary and secondary metabolites. Metabolomics is an important tech

nique for deciphering the unknown metabolites or quantification of known

metabolites in plants formed on being exposed to such limiting factors.

Metabolome refers to the total metabolite constituent of any plant species

that can be analyzed to further explore the changes that take place in plants

on being exposed to environmental stresses. Metabolomics is a promising

tool that helps in the proper identification of metabolic networks linked with

abiotic stress that might help in the identification of stress-specific marker

in plants. Advanced metabolomics tools such as non-destructive nuclear

magnetic resonance (NMR), gas chromatography-mass spectrometry

(GS-MS), liquid chromatography-mass spectrometry (LC-MS) and direct

flow injection mass spectrometry have enhanced the rate of metabolome

profiling of plants. Presently, integration of metabolomics with other omics